| CXIDB ID 171 | |
| Deposition Summary | |
|---|---|
| Depositor: | Keitaro Yamashita |
| Contact: | [email protected] |
| Deposition date: | 2020-10-21 |
| Last modified: | 2020-11-05 |
| DOI: | 10.11577/1679953 |
| Associated PDB: | 7CJ3 |
| Publication Details | |
| Title: | Structural insights into the mechanism of rhodopsin phosphodiesterase |
| Authors: | Tatsuya Ikuta et al. |
| Journal: | Nature Communications |
| Year: | 2020 |
| DOI: | 10.1038/s41467-020-19376-7 |
| Experimental Conditions | |
| Method: | Synchrotron Crystallography (small-wedge) |
| Sample: | RhPDE-TMD |
| Wavelength: | 1.00 Å (12.4 keV) |
| Lightsource: | SPring-8 |
| Beamline: | BL32XU |
Data Files
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| Auxiliary Files | |
| Raw Data: | 180608_BL32XU/ (4 KB) |
| Raw Data: | 180803_BL32XU/ (4 KB) |
Description
Crystal structures of TMD of Rhodopsin phosphodiesterase (PDB code: 7CJ3). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.
